can be recovered from other clinical specimens including CSF, abscess material, respiratory secretions, amniotic fluid, the urogenital tract, joint fluid, and various tissues. 40 Performing a Gram stain on blood culture sediment at the first indication of growth can yield a presumptive identification, although there is a small chance that the Gram stain can be misread. appear to be sensitive to the anticoagulant sodium polyanethole sulfonate used in some blood culture bottles. 64 Recovery of capnocytophagae from CSF or blood typically takes 3 to 7 days. Clinical suspicion of Capnocytophaga septicemia should prompt a request to the laboratory to incubate blood cultures for up to 10 days or longer to enhance recovery of this fastidious, often slow-growing organism. 63ĭespite the widespread application of molecular techniques to identify organisms from clinical samples, most clinically significant isolates of Capnocytophaga are detected by culture.
61 16S ribosomal RNA (rRNA) gene sequencing performed directly on a clinical sample or a positive blood culture or CSF can decrease the turnaround time to diagnosis, 59,62 and polymerase chain reaction (PCR) assay using species-specific primers can detect Capnocytophaga in mixed culture in dental plaque samples. Pers and colleagues 60 reported seeing bacteria on initial microscopic examinations of cerebrospinal fluid (CSF), whereas another report described slender, fusiform bacilli in conjunctival scrapings. 59 The presence of Capnocytophaga should be strongly suspected if slender, medium to long, gram-negative rods with tapered ends are observed ( Fig. Presumptive diagnosis of Capnocytophaga sepsis can be made by examination of the patient's peripheral whole blood or buffy coat using Gram or Wright-Giemsa stain. Bennett MD, in Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases, 2020 Diagnosis and Laboratory Identification It does not store any personal data.John E. The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. The cookie is used to store the user consent for the cookies in the category "Performance". This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Other.
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